Proline hydroxylation and gene expression

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August undefined, 2024

WebAug 3, 2024 · Hydroxylation of proline residues by proline hydroxylase domain enzymes (PHDs) is a key step in the degradation of the α-subunit (32,35,36): ... specific deletion of the HIF-1α gene in mice exacerbates alcoholic hepatic steatosis and causes alterations in hepatic gene expression, ... WebOct 6, 2024 · Proline hydroxylation targets both ectopically expressed ChREBP in cells and endogenous ChREBP in mouse liver. Functionally, we found that specific hydroxylated prolines were dispensable for...

Proline hydroxylation and gene expression

WebSep 10, 2024 · Analysis of the relative gene expression data was con-ducted using the 2 −ΔΔCt method . 3. Results 3.1. Genome-Wide Identification of 2OGD Members in Brassica Plants ... Effective hydroxylation of proline-rich, collagen-like, and hypoxia-inducible transcription factor alpha-like peptides. J. Biol. Chem. 2002, 277, 23965–23971. WebDescription: Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 3, mRNA. (from RefSeq NM_001243084) RefSeq Summary (NM_001243084): This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a … company house hikma

Hydroxylation of HIF-1: Oxygen Sensing at the Molecular Level

WebPHD2 was significantly overexpressed in AML patients, which shows that PHD2-mediated proline hydroxylation is a potential oncogenic pathway in AML, driving BRD4-mediated gene expression and cancer ... WebMar 2, 2024 · Proline hydroxylation can be observed by the mass increments of 16 Da in single charged ions (or 8 and 5.3 Da in double and triple charged ions, respectively). ... Stable expression of the gene silencing constructs or a complete knockout of Nb-P4H genes may completely prevent Hyp formation. WebJul 7, 2005 · DeepDyve PROLINE HYDROXYLATION AND GENE EXPRESSION Kaelin, William G. Annual Review of Biochemistry, Volume 74 – Jul 7, 2005 Read Article Download PDF … company house highbridge resourcing

Role and regulation of prolyl hydroxylase domain proteins

Proline-dependent regulation of collagen metabolism

WebJan 1, 2005 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy. WebJul 3, 2003 · Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. eawards uspsWebTherefore, the relative gene expression levels of the MRFs gene and its crucial positive and negative regulatory signals (IGF-I and MSTN) were analyzed by q-PCR in this study. Compared with FM, transcription of Myod , Mrf5 , Myog , and IGF-I was significantly down-regulated and MSTN was significantly up-regulated in SBM, PM, CM, SEM, and CGM ... e awards vcb

"WebAug 28, 2014 · Hypoxia-inducible factor (HIF) is a master regulator that mediates major changes in gene expression under hypoxic conditions. Though HIF family has been identified in many organisms, little is known about this family in … " - Proline hydroxylation and gene expression

Proline hydroxylation and gene expression

WebProline hydroxylation also plays a unique role in mediating the cellular response to oxygen through hypoxia inducible factor-1 (HIF-1). Under hypoxic conditions, HIF-1α/HIF-1β heterodimers induce expression of ... Asn803 of HIF. 90 This hydroxylation event prevents the recruitment of transcriptional coactivators p300 and CBP and gene ... WebDec 31, 2010 · The proline hydroxylation occurs at the γ-C atom, forming hydroxyproline, which is an essential element of collagen, and can stabilize the triple helix structure in turn a necessary element of collagen protofibrils. ... Kaelin WG (2005) Proline hydroxylation and gene expression. Annu Rev Biochem 74: 115–128. View Article Google Scholar 3. ...

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WebAug 17, 2012 · Recombinant expression requires proline hydroxylation, a post-translational modification which is critical for imparting stability and structure. ... Leigh SD, Fong BE, Chang R, Daniels GA, et al: Production of recombinant human type I procollagen trimers using a four-gene expression system in the yeast Saccharomyces cerevisiae. J Biol Chem ... WebNov 8, 2024 · Request PDF Histone H3 proline 16 hydroxylation regulates mammalian gene expression Histone post-translational modifications (PTMs) are important for regulating …

WebJul 26, 2024 · Proline hydroxylation and collagen fibril formation Experiments utilising recombinant collagen produced in insect cells, yeast and plants have indicated that collagen molecules lacking 4-Hyp cannot form triple helices that are stable at a physiological temperature [ 36–38 ]. WebThe biocontrol agent Lysobacter enzymogenes OH11 produces several structurally distinct antibiotic compounds, including the antifungal HSAF (Heat Stable Antifungal Factor) and alteramides, along with their 3-dehydroxyl precursors (3-deOH). We previously showed that the 3-hydroxylation is the final step of the biosynthesis and is also a key structural moiety …

WebNov 29, 2016 · Proline hydroxylation (Hyp) is a key oxygen-sensing posttranslational modification that is dynamically modulated during tumor cell proliferation and … WebProline hydroxylation and gene expression Hypoxia-inducible factor (HIF) is a master transcriptional regulator of hypoxia-inducible genes and consists of a labile alpha subunit (such as HIF1alpha) and a stable beta subunit (such as HIF1beta or ARNT). In the …

WebAbstract. Abstract Hypoxia-inducible factor (HIF) is a master transcriptional regulator of hypoxia-inducible genes and consists of a labile α subunit (such as HIF1α) and a stable β …

Webthe proline hydroxylation path-way in regulating BRD4-medi-ated protein-protein interaction and gene expression. Graphical Abstract Highlights • BRD4 interaction with prolyl hydroxylase domain proteins. • Stoichiometric dynamics and site-specific regulation of BRD4 proline hydroxylation. eawards uokWebJun 10, 2013 · Kaelin WG: Proline hydroxylation and gene expression. Annu Rev Biochem. 2005, 74: 115-128. 10.1146/annurev.biochem.74.082803.133142. Article CAS PubMed Google Scholar Wang GL, Jiang BH, Rue EA, Semenza GL: Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. eaw as460eWebJan 1, 2005 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy. eawards upsiWebNov 9, 2024 · This study characterizes a regulatory mark in the histone code and reveals a role for H3P16oh in regulating mammalian gene expression. Publication Liu, X., Wang, J., … eaw as660i eaw array calculatorWebHistone post-translational modifications (PTMs) are important for regulating various DNA-templated processes. Here, we report the existence of a histone PTM in mammalian cells, … eaw as490WebCollagen, an essential part of connective tissue, forms 25%–35% of the proteins in our bodies. The hydroxylation occurs at the γ-Carbon atom of Proline, forming hydroxyproline (Hyp), which is an essential component of collagen occurring at every 3rd residue in its amino acid sequence. In some instances, hydroxylation of Proline at its β ... eaw as660e