Circrna pulldown probe

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August undefined, 2024

WebOct 21, 2024 · Probe sequences were listed in the Major Resources Table in the Data Supplement. Statistical Analysis. All statistical analyses were performed using the GraphPad Prism software, version 8. ... . circRNA pull-down assay using biotin-labeled circSOD2 BSJ RNA probe followed by RT-qPCR identified 3 miRNA candidates that potentially bind … WebDec 16, 2024 · The probe contains four repeats allowing efficient pulldown of the circRNA Compatible with downstream assays, including RNAseq and proteomics Publisher’s …

CircMUC16 promotes autophagy of epithelial ovarian cancer via ...

WebSep 1, 2024 · To pull down the miRNA by circRNA, biotinylated-circSMARCC1 probe (5′-CATCTTCCTCATCACAGCAC-3′) was synthesized by RiboBio (Guangzhou, China), and the oligo probe (5′-TATGTTGTTGATTTGCTGGC-3′) was used as a control. CircRNA pulldown assay was carried out using PierceTM Magnetic RNA-Protein Pull-Down Kit … WebThe intracellular transfer of circEDIL3 by SMSCs-Exos may be a potential novel therapeutic strategy for Rheumatoid Arthritis. ios how do i turn my ringer volume down

CircRNAs: a new target for the diagnosis and treatment of ... - Nature

WebJul 20, 2024 · Circular RNAs (circRNAs) are a large family of noncoding RNA molecules that have emerged as novel regulators of gene expression by sequestering microRNAs … WebAbnormal levels or dysregulation of miRNA or circRNA are linked to several cancerous pathologies, so that they are receiving a large attention as diagnostic and prognostic tools. ... and the Atto390 dye, bound to the 5′ end of miR4749-5p, provides the acceptor (A), with these probes representing a very appropriate D-A couple for FRET . The ... WebFeb 28, 2024 · CircRNA pulldown. The specific steps of circRNA pulldown are described in our previous study . ... Cy3-labelled probe against circMUC16 (5’CY3-TCTTTCTCGGGCCTGAGCAAGGTCAGT- 3’CY3) and FITC-labeled probe against miR-199a were obtained from Genepharma. The FISH assay was performed following the … ios house style

CircMUC16 promotes autophagy of epithelial ovarian cancer via ...

Circular RNA hsa_circ_0005218 promotes the early recurrence of ...

WebThe probe was synthesized by Bersinbio (Guangzhou, China). ... Identification of circ_0005218 as an up-regulated circRNA. (A) scatter, (B) ... Mechanically, through RNA pull-down, dual-luciferase reporter and rescue assays, we showed that circ_0005218 could serve as a sponge of miR-31-5p to up-regulate the CDK1 expression level, thus … WebJul 19, 2024 · Circular RNAs (circRNAs), a recently identified new member of non-coding RNAs, are demonstrated to participate in diverse biological processes, such as … on this day in history 1078WebFeb 18, 2024 · The biotinylated circRNA-CDR1as probe was designed to specifically bind to the junction area of circRNA-CDR1as and a random oligo was used as control probe. About 3 μg of probes were incubated with ~ 1 × 10 7 cells that had been washed with ice-cold PBS and lysed in a lysis buffer for 2 h at room temperature. ios how to change default image to tabbar

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Circrna pulldown probe

RNA Pulldown - Creative Biogene IntegrateRNA

http://www.circrna.com.cn/?p=4923 WebSep 9, 2024 · RNA pull down and mass spectrometry Biotin-labeled oligonucleotide probes targeting junction sites of circRNAs were synthesized by SunBio Biomedical Technology Co., Ltd (Shanghai, China)....

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WebJan 1, 2015 · After incubation of LOC100506178 probes with streptavidin-coated magnetic beads, ... The RNA pull-down assay was adapted from previously published studies [27] or protocols [28]. Unedited and ... WebOct 25, 2024 · RNA pulldown and luciferase assays were performed to investigate the interaction between circular CD44 (circCD44, also annotated as hsa_circ_0021735) and miR-502–5p. RNA pulldown and RIP assays were used to investigate the interaction between circCD44 and IGF2BP2.

WebThe RNA pulldown assay selectively extracts protein-RNA complexes from the sample. In general, RNA pulldown assays take advantage of high-affinity tags (such as biotin, etc.) The RNA probe can be biotinylated to complex with proteins in the cell lysate and then purified using agarose or magnetic beads. Alternatively, the protein can be labeled ... WebFeb 4, 2024 · CircRNA has emerged as a new non-coding RNA that plays crucial roles in tumour initiation and development. ‘MiRNA sponge’ is the most reported role played by circRNAs in many tumours.

WebJul 19, 2016 · 环状RNA研究方法之RNA Pull-Down. 环状RNA（circular RNA，circRNA）是近来研究很热门的一种特殊的长链非编码RNA。. 研究发现，circRNA 在人体细胞中广泛 … WebJun 2, 2024 · A novel and highly specific circRNA-pulldown followed by mass spectrometry analysis identified 94 circFOXK2-interacting proteins, which were involved in cell adhesion, mRNA splicing, and structural molecule activity. Of these, circFOKX2 interactions with YBX1 and hnRNPK enhanced expression of oncogenes NUF2 and PDXK.

WebcircRNA Pull-down Assay. IntegrateRNA is an outstanding biotechnology company with years of experience in RNA-protein interaction analysis. We offer customized circRNA …

Web新一代circRNA pull-down技术路线. 本项技术在吉赛生物circRNA过表达载体技术基础上，将RNA标签体系引入circRNA中，实现在细胞内表达带有特异性标签的circRNA；通过RNA标签与捕获蛋白的高度特异且稳定的相 … ios how to add widgetWebFeb 26, 2024 · A biotin-labeled circRAB11FIP1 probe was provided by GenePharma. ... Then, qPCR was used to determine miRNA expression. CircRNA pulldown. The circRNA pulldown was performed as previously described ... ios how to call services in launch screenWebOct 22, 2024 · Circular RNAs (circRNAs) are a covalently enclosed endogenous biomolecules, which has tissue specificity and cell specificity in eukaryotes, and are produced after being regulated by specific cis... on this day in history 1069WebDec 7, 2024 · For circRNA pulldown experiments, a dedicated biotin-labeled circDOCK1 probe was used, and circDOCK1-related RNAs were purified prior to qRT–PCR. Based on the experimental results, compared with control group samples, samples pulled down with the circDOCK1-specific probe showed notable enrichment of only miR-339-3p (Fig. 3F). on this day in history 1089WebApr 11, 2024 · Nuclear-cytoplasmic fractionation and FISH were used to identify the localization of circRNA and miRNA. Pull-down, RIP, and luciferase activity assays were performed to show the biding ability of circRNA, miRNA and target proteins. ... Target Prove is BaseScope Probe-BA-Hs-PALM-E7E2-circRNA (Advanced Cell Diagnostics China, … on this day in history 1084WebJan 31, 2024 · The prediction of circRNA functions is instrumental to interpret their impact in diseases, and to prioritize circRNAs for functional investigation. Currently, circRNA functional predictions are provided by web databases that do not allow custom analyses, while self-standing circRNA prediction tools are mostly limited to predict only one type of ... on this day in history 1627WebJun 29, 2024 · CircRNA pulldown was performed using Magnetic RNA-protein Pull-down Kit (Thermo), according to the manufacturer’s protocol. 5′Biotin-labeled oligonucleotide probe targeting junction site of circSHKBP1 was synthesized (KeyGEN, Jiangsu, China): 5′-CCTCCTGGACTGGTCTTGGG-3′. on this day in history 12